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Standard curves generated using <t>gBlocks</t> Gene Fragments <t>GEO</t> 1/2007 (GEO 1/2007-P1192R in the case of LAMP2) by two LAMP assays—LAMP1 and LAMP2 (top panel) and three real-time PCR assays—WOAH-King, WOAH-Fernandez-Pinero, and USDA-Zsak (bottom panel).
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Standard curves generated using <t>gBlocks</t> Gene Fragments <t>GEO</t> 1/2007 (GEO 1/2007-P1192R in the case of LAMP2) by two LAMP assays—LAMP1 and LAMP2 (top panel) and three real-time PCR assays—WOAH-King, WOAH-Fernandez-Pinero, and USDA-Zsak (bottom panel).
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Standard curves generated using <t>gBlocks</t> Gene Fragments <t>GEO</t> 1/2007 (GEO 1/2007-P1192R in the case of LAMP2) by two LAMP assays—LAMP1 and LAMP2 (top panel) and three real-time PCR assays—WOAH-King, WOAH-Fernandez-Pinero, and USDA-Zsak (bottom panel).
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Standard curves generated using <t>gBlocks</t> Gene Fragments <t>GEO</t> 1/2007 (GEO 1/2007-P1192R in the case of LAMP2) by two LAMP assays—LAMP1 and LAMP2 (top panel) and three real-time PCR assays—WOAH-King, WOAH-Fernandez-Pinero, and USDA-Zsak (bottom panel).
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Standard curves generated using gBlocks Gene Fragments GEO 1/2007 (GEO 1/2007-P1192R in the case of LAMP2) by two LAMP assays—LAMP1 and LAMP2 (top panel) and three real-time PCR assays—WOAH-King, WOAH-Fernandez-Pinero, and USDA-Zsak (bottom panel).

Journal: Frontiers in Veterinary Science

Article Title: Development of a novel p72 gene-based loop-mediated isothermal amplification assay for the rapid detection of African swine fever virus in animal feed

doi: 10.3389/fvets.2025.1681069

Figure Lengend Snippet: Standard curves generated using gBlocks Gene Fragments GEO 1/2007 (GEO 1/2007-P1192R in the case of LAMP2) by two LAMP assays—LAMP1 and LAMP2 (top panel) and three real-time PCR assays—WOAH-King, WOAH-Fernandez-Pinero, and USDA-Zsak (bottom panel).

Article Snippet: Briefly, the assay LODs were determined initially using 10-fold serial dilutions (ranging from 10 6 to 10 0 copies/reaction) of the gBlocks Gene Fragment GEO 1/2007 for the ASFV Georgia strain p72 gene (GEO 1/2007- P1192R for the ASFV Georgia strain topoisomerase II gene for LAMP2), ranging from 10 6 to 10 0 copies/reaction, and Quantitative Synthetic ASFV DNA ATCC VR-3283SD, ranging from 8.8 × 10 5 to 0.8 copies/reaction.

Techniques: Generated, Real-time Polymerase Chain Reaction